I have lost 41 pounds so far am taking a multi vitamin, omega 3 and biotin. Nothing has helped so far. I also get b-6 and b-12 shots. My hair is falling out in hand fulls.

Given the supplements you mentioned, chances are that you're not eating enough protein. Hair strands are mainly composed of protein and if your diet is insufficient in protein, hair starts falling out.
Good sources of protein are eggs (whole, not just the whites - rich in biotin, sulphur, choline and B vitamins. Believe it or not 1 or 2 a day won't affect your cholesterol levels much, but just don't overdo it), meat (go for lean meats), fish (tuna, salmon, etc.) dairy (go for skim or low-fat), and nuts (i.e. almonds, but watch your portions! 1/4 cup/a handful is the standard portion size). Shoot for 50-70g of protein a day.
Also, try eating a LITTLE bit more fat everyday. Healthy fat, of course - avocados, nuts and nut butters (i.e. peanut butter), olive oil, etc. This will help keep your hair healthy too (but it doesn't mean you should go overboard).
Good luck, and congratulations on your weight loss.

Joan, rapid weight loss can cause hair loss. In your case, it could be due to that, or something else - such as hypothyroidism, stress etc. It could be nutrient deficiency or poor hair care. Anyway, you can read all about these here:
http://www.hairlossmentor.com
If everything that is written (in the website) fits you to a T and you are still losing hair, then I suggest you go and see a dermatologist or doctor. Good luck.